Trichloroacetic acid, for electrophoresis, suitable for fixing solution (for IEF and PAGE gels), ≥99% ยี่ห้อ Sigma

PROPERTIES

vapor density

<1 (vs air)

Quality Level

200

vapor pressure

1 mmHg ( 51 °C)

Assay

≥99%

form

powder or flakes (or crystals)

technique(s)

electrophoresis: suitable

turbidity

≤3 NTU (1 g/ml)

refractive index

n20/D 1.62 (lit.)

bp

196 °C (lit.)

mp

54-58 °C (lit.)

solubility

water: 1 g/mL, clear, colorless

density

1.62 g/mL at 25 °C (lit.)

anion traces

chloride (Cl^-): ≤0.001%

nitrate (NO₃^-): ≤0.002%

phosphate (PO₄^3-): ≤5 ppm

sulfate (SO₄^2-): ≤0.02%

cation traces

Fe: ≤0.0005%

heavy metals (as Pb): ≤0.002%

suitability

suitable for fixing solution (for IEF and PAGE gels)

application(s)

cell analysis

storage temp.

2-8°C

SMILES string

OC(=O)C(Cl)(Cl)Cl

InChI

1S/C2HCl3O2/c3-2(4,5)1(6)7/h(H,6,7)

InChI key

YNJBWRMUSHSURL-UHFFFAOYSA-N

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DESCRIPTION

General description

Trichloroacetic acid (TCA) is produced during the chlorination of water comprising natural organic material.[1] It is a novel reagent used in clinical laboratories for deproteinization of body fluids.[2] In addition, TCA is also used in the treatment of genital warts[3] and acne scars.[4]

Application

Trichloroacetic acid has been used:

·       for hydrolysis of DNA isolated from the cell[5]

·       for precipitation of glutathione isolated from seminal plasma[6]

·       as a fixative in microscopy[7]

Traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. Used as decalcifier and fixative in microscopy.