Glutamine and Glutamate Determination Kit ยี่ห้อ Sigma

PROPERTIES

usage

1 kit sufficient for 50 tests

Quality Level

200

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

UV/Vis spectroscopy: suitable

λmax

340 nm

application(s)

cell analysis

detection

storage temp.

2-8°C

DESCRIPTION

Application

For enzymatic determination of L-glutamine and L-glutamate via reduction of NAD to NADH, measured spectrophotometrically at 339 nm.

The detection ranges for this kit are: 0.056 - 0.56 mM L-glutamate; 0.14 - 1.4 mM L-glutamine.

Glutamine and Glutamate Determination Kit has been used to determine the concentration of L-glutamine:

in minimum essential medium (MEM) medium[1]

in the supernatant[2]

in plasma[3]

Biochem/physiol Actions

The Glutamine/Glutamate Determination Kit is designed for the spectrophotometric measurement of L-glutamine and/or L-glutamate via enzymatic deamination of L-glutamine and dehydrogenation of L-glutamate with conversion of NAD+ to NADH. This kit may be used for the spectrophotometric measurement of L-glutamine and/or L-glutamate in liquid preparations.