Bradford Reagent, for 1-1,400 μg/ml protein ยี่ห้อ Sigma

PROPERTIES

Quality Level

100

200

form

solution

storage temp.

2-8°C

DESCRIPTION

General description

Bradford assay is addition of coomassie brilliant blue G-250 to protein solution. The coomassie blue dye associates with basic and aromatic amino acids, thereby causing shift in absorbance during protein determination.[1]

Application

Bradford Reagent has been used to determine total protein concentration.[2][3][4]

Features and Benefits

·       The reagent is ready to use. No mixing or dilution required.

·       Color development is rapid. Only a five minute incubation and then the sample is read a 595 nm.

·       Reducing sugars and reducing substances along with thiols do not interfere with this reagent.

·       Reagent is suitable for micro (1-10 μg/ml) and standard (50-1400 μg/ml) assays.

·       Can be used in microwell plate assays.

·       Inexpensive assay.

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