repliQa HiFi ToughMix, 100R ยี่ห้อ Quantabio

Features & Benefits

• High Fidelity – >90x wild type Taq
• Extreme Speed – up to 3x faster PCR results with extension rates as fast as 1 kb/sec*
• Tough Tested – tolerant to a wide range of PCR inhibitors
• Superior Sensitivity – higher yields from lower inputs as little as 2 pg
• Long Range – amplify +24 kb gDNA, +40 kb λDNA

Description

The repliQa HiFi ToughMix is a 2x, ready-to-use master mix that contains all the components for high fidelity PCR amplification, including a genetically modified DNA polymerase coupled with hot start antibodies. This unique, next generation master mix provides 90x higher fidelity compared to Taq, while reducing time to PCR results by 2-3x. The extreme speed is enabled by extension times as fast as 1-10 kb/sec depending on target length. The enzyme is coupled with the industry leading ToughMix which is tolerant to a wide variety of inhibitors making it suitable for routine PCR, cloning, amplicon sequencing and site directed mutagenesis.

Contents

2x reaction buffer containing optimized concentrations of MgCl₂, dNTP’s and proprietarily formulated HiFi polymerase, hot start antibodies and ToughMix chemistry

Storage & Handling

Store kit components in a constant temperature freezer at -25°C to -15°C upon receipt.

For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.