QuantiNova PCR Kits

For highly sensitive, specific and ultrafast probe-based real-time PCR and multiplex PCR, and unparalleled results using SYBR Green-based qPCR

Features

• Precise reaction setup with a built-in visual indicator
• Accurate and robust detection of targets down to one copy
• Unmatched specificity due to novel hot-start mechanism
• Sensitive detection of up to 5 targets in 1 tube
• Reaction stability of up to 100 hours at 30°C for flexible workflows

QuantiNova Probe PCR Kit (100)

Cat. No. / ID: 208252

For 100 x 20 µl reactions: 1 ml 2x QuantiNova Probe PCR Master Mix , 250 µl QN ROX Reference Dye, 500 µl QuantiNova Yellow Template Dilution Buffer, 1.9 ml Water

Product Details

Featuring enhanced specificity, sensitivity, speed, and process safety, the QuantiNova SYBR Green PCR Kit (SYBR qPCR kit) sets new standards in SYBR Green-based qPCR. A novel hot-start mechanism significantly increases the specificity of real-time PCR and optimized chemistry produces accurate quantitative results from cDNA or gDNA on any real-time PCR cycler with detection of even a single target copy. Finally, a built-in visual indicator ensures correct pipetting.

Avoid the deleterious effects of mispriming at lower temperatures with the QuantiNova Probe PCR Kit (probe qPCR kit). Based on a novel, antibody-mediated, hot-start mechanism, the kit enhances the specificity and efficiency of probe-based real-time PCR to provide accurate, singleplex or duplex, cDNA or gDNA analysis on various real-time PCR cyclers. The added convenience of extreme stability for up to 100 hours at room temperature, without a need for any cooling agent, makes it ideal for handling of high-throughput samples and automated workflows. An in-built tracking system for visual identification of correct pipetting gives you absolute peace of mind while extremely sensitive detection of even low target amounts ensures reliable qPCR results every time.

QuantiNova Multiplex PCR Kits (multiplex qPCR kits) enable fast and reliable quantification of up to 5 cDNA or gDNA targets in a single tube by multiplex, real-time PCR or two-step RT-PCR. Q-bond technology and an optimized master mix promote ultrafast multiplex real-time PCR within 1 hour. The combination of a unique hot start and PCR buffer system in the ready-to-use 4x master mix ensures highly sensitive qPCR on any real-time cycler without the need for optimization, also providing automated reaction set up options at room temperature. An in-built tracking system for visual identification of correct pipetting helps to prevent human errors, and the combination with QuantiNova Reverse Transcription Kit for cDNA synthesis allows inclusion of QuantiNova Internal Control RNA to monitor successful reverse transcription and qPCR.