ProteoSilver™ Plus Silver Stain Kit ยี่ห้อ Sigma

PROPERTIES

usage

1 kit sufficient for 25 mini-gels (10 × 10 cm)

Quality Level

200

technique(s)

protein staining: suitable

storage temp.

20-25°C

DESCRIPTION

General description

Conveniently packaged, extremely stable, and highly sensitive, ProteoSilver^™ Plus Silver Stain Kit is an ideal product for any protein scientist. Similar to ProteoSilver (PROTSIL1), this kit contains prepared solutions of silver staining reagents along with detailed instructions to achieve optimal results. With a detection limit of 0.1 ng/mm² of protein (BSA) and an extremely low background, ProteoSilver Plus leads to superior detection of low abundance proteins.

ProteoSilver Plus is also MALDI-MS compatible and does not contain glutaraldehyde, which crosslinks lysine residues, resulting in inaccurate spectra. ProteoSilver Plus Silver Stain Kit contains two additional reagents for destaining an excised protein spot, for those wishing to perform further characterization through tryptic digestion and MALDI-MS analysis.

Application

ProteoSilver^® Silver Stain Kit has been used for staining of the gels for visualizing proteins. It has also been used for destaining of the protein of interest using destainer provided in the kit for mass spectroscopic analysis.[1][2][3]

Features and Benefits

·       Premixed and preweighed solutions reduce time and cost of purchasing and preparing individual components

·       Optimized protocols help establish conditions for best results

·       High sensitivity and low background ensure very low abundance proteins can be detected and resolved from other proteins

·       MALDI Compatible - Protein spots of interest can be further characterized by mass spectrometry

·       Room Temperature Stability allows easy and convenient storage

Legal Information

ProteoSilver is a trademark of Sigma-Aldrich Co. LLC

Sigma-Aldrich is a registered trademark of Sigma-Aldrich Co. LLC