Multi Species Phosphorylated Neurofilament ELISA Kit ยี่ห้อ Sigma

DESCRIPTION

Application

Research Category

Neuroscience

The Phosphorylated Neurofilament H (pNF-H) kit is a sandwich enzyme immunoassay (EIA), which measures pNF-H.

Used to detect/quantify: Phosphorylated Neurofilament (pNF-H)

Packaging

96 wells

Components

Immunoplate Pre-coated with Chicken anti-pNF-H Polyclonal Antibody: (Part No. 2006263). One 96-well immunoplate pre-coated with chicken anti- pNF-H polyclonal antibody sealed in a foil pouch.

Rabbit anti- pNF-H Polyclonal Antibody: (Part No. 2006919). One vial (100 μL) of rabbit anti- pNF-H polyclonal antibody.

Goat anti-Rabbit, Alkaline Phosphatase Conjugated Antibody: (Part No. 2006917). One vial (10 μL) of goat anti-rabbit alkaline phosphatase conjugate.

Bovine pNF-H Standard: (Part No. 2006918). One vial (5.0 ng) of bovine pNF-H standard.

20X TBS: (Part No. 2004913). One bottle (50 mL) of 20X TBS.

20% Tween^® 20: (Part No. 2004915). One vial (3 mL) of 20% Tween-20.

Elisa Assay Diluent: (Part No. 2006921) One bottle (30 mL) of Elisa assay diluent containg 0.05% Kathon as a preservative, provided in a ready to use format.

pNPP Buffer: (Part No. 2006261). One bottle (12 mL) of pNPP buffer for use with 50X p-NPP, provided in a ready to use format.

50X pNPP: (Part No. 2006262). One vial (250 μL) of 50X pNPP (p-nitrophenyl phosphatase) alkaline phosphatase substrate.

Plate Covers - Two Plate Covers.

Storage and Stability

Prior to use maintain the kit components at 2°-8°C and -20°C, as indicated, until expiration date.

1. Wash Buffer - After dilution maintain the Wash Solution at 2°-8°C for up to one month or until expiration date.

2. Tween^® 20 - Store at room temperature upon receipt of kit.

3. Elisa Assay Buffer - Aliquot as required to avoid repeated freeze-thaw cycles. After thawing, maintain the Elisa Assay Buffer at 2°-8°C for up to one month, or until expiration date.

4. Immunoplate Pre-coated with Chicken anti- pNF-H Polyclonal Antibody - Unused strip wells may be returned to the foil pouch and stored at 2°-8°C for up to one month, or until expiration date.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

Disclaimer

For research use only. Not for use in diagnostic procedures.