Pyruvate Dehydrogenase Activity Assay Kit ยี่ห้อ Sigma

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General description

Pyruvate Dehydrogenase (PDH) is a mitochondrial enzyme which has an important role in carbohydrate metabolism. Combining with dihydrolipoyl transacetylase (E2) and dihydrolipoyl dehydrogenase (E3), PDH (E1) forms a well-characterized enzyme complex (PDC). In the presence of NAD⁺ and CoA, PDH catalyzes the conversion of pyruvate into Acetyl-CoA and CO₂. Thus, PDH links the glycolysis metabolic pathway (cytoplasm) to the citric acid cycle (mitochondria). The Pyruvate Dehydrogenase Activity Assay Kit provides a sensitive, simple, fast, direct and convenient way for the detection of PDH activity in various samples, based on a colorimetric method. In the assay, PDH activity changes a colorless developer to a colored product. The amount of the colorimetric (450 nm) product is proportional to the enzymatic activity present in the sample. The kit can detect PDH activity that is lower than 0.1 mU, in a variety of samples.

Application

Pyruvate Dehydrogenase (PDH) Activity Assay Kit has been used in the colorimetric measurement of PDH Activity in biological samples.

Features and Benefits

Sensitive, simple, fast, direct and convenient way for the detection of PDH activity in various biological samples.

Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.

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Suitability

Suitable for measuring PDH Activity in adherent or suspension cells, from an animal or plant source.

Unit Definition

One unit of pyruvate dehydrogenase is the amount of enzyme that will generate 1.0 µmole of NADH per minute at 37 °C at pH=7.5.