Alkaline Phosphatase (ALP) Assay Kit ยี่ห้อ Sigma

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General description

Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hyperalkalinephosphatasemia, has been associated with malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma and sarcoidosis.

Features and Benefits

·      Detection limit of 0.02 U/L

·      Compatible with high-throughput handling systems.

Suitability

The kit is suitable for ALP activity in serum, plasma and other sources, characterization and quality control for ALP production, and for high-throughput screening for ALP modulators.

Principle

The Alkaline Phosphatase (ALP) Assay Kit utilizes 4-methylumbelliferyl phosphate that is hydrolyzed by ALP into the highly fluorescent product 4methylumbelliferone. The rate of the fluorescence increase is directly proportional to the enzyme activity.