Glutamate Dehydrogenase (GDH) Assay Kit ยี่ห้อ Sigma

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General description

Glutamate dehydrogenase (GLDH) is an enzyme which catalyzes the interconversion of glutamate and α-ketoglutarate. Elevated blood serum GLDH levels indicate liver damage; thus, GLDH plays an important role in the study of liver disease, especially in combination with aminotransferases. Transgenic plants expressing microbial GLDHs are improved in tolerance to herbicide, water deficit, and pathogen infections.

Features and Benefits

·      Linear detection range: 0.4 to 80 U/L for 30 minute reaction. Detection Limit of 0.1 U/L for 120 minute reaction.

·      Compatible with high-throughput handling systems.

Suitability

The kit is suitable for glutamate dehydrogenase activity determination in biological samples (e.g. plasma, serum, urine, tissue, and culture media.)

Principle

The non-radioactive, colorimetric Glutamate Dehydrogenase (GDH) Assay Kit is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.