NADP/NADPH Assay Kit ยี่ห้อ Sigma

คำอธิบาย

General description

Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+ /NADPH has applications in research pertaining to energy transformation and redox state of cells or tissue.

Features and Benefits

·      The linear detection range of the kit is 0.1 µM to 10 µM NADP+ /NADPH

·      Compatible with high-throughput handling systems.

·      Convenient, the procedure involves adding a single working reagent, and reading the optical density at time zero and 30 minutes at room temperature.

Suitability

The kit is suitable for detection of NADP+ /NADPH concentrations and ratios in cell or tissue extracts.

Principle

The NADP/NADPH Assay Kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportionate to the NADP+ /NADPH concentration in the sample. This assay is highly specific for NADP+ /NADPH and is not interfered by NAD+ /NADH.