Glutamate Oxidase Assay Kit, ยี่ห้อ Sigma

PROPERTIES

usage

sufficient for 200 fluorometric tests

detection method

fluorometric

storage temp.

−20°C

DESCRIPTION

General description

Glutamate oxidase is an oxidoreductase that catalyzes the oxidative deamination of L-glutamate to ketoglutarate, ammonia and hydrogen peroxide.

Glutamate oxidase is an oxidoreductase that catalyzes the oxidative deamination of L-glutamate to ketoglutarate, ammonia, and hydrogen peroxide.

The Glutamate Oxidase Assay Kit provides a simple and direct procedure for measuring glutamate oxidase activity in a variety of samples such as solutions and cell extracts. Glutamate Oxidase activity is determined by a coupled enzyme assay, in which the oxidation of glutamic acid results in a fluorescent product (λex =540/λem = 590 nm) proportional to the glutamate oxidase activity present. One unit of glutamate oxidase activity is defined as the amount of enzyme that catalyzes the conversion of L-glutamic acid to 1.0 μmole a-ketoglutaric acid per minute at pH 7.4 at room temperature.

Suitability

Suitable for measuring glutamate oxidase activity in a variety of samples such as solutions and cell extracts.

Principle

The glutamate oxidase assay kit provides a simple and direct procedure for measuring glutamate oxidase activity in a variety of samples such as solutions and cell extracts. Glutamate oxidase activity is determined by a coupled enzyme assay, in which the oxidation of glutamic acid results in a fluorescent product (λex =540/λem = 590 nm) proportional to the glutamate oxidase activity present. One unit of glutamate oxidase activity is defined as the amount of enzyme that catalyzes the conversion of L-glutamic acid to 1.0μmole α-ketoglutaric acid per minute at pH 7.4 at room temperature.