Multidrug Resistance Assay Kit ยี่ห้อ Sigma

PROPERTIES

usage

(Fluorometric MDR Assay)

detection method

fluorometric

storage temp.

−20°C

Gene Information

human ... ABCB1(5243) , ABCC2(1244)

mouse ... ABCC2(12780)

rat ... abcc2(25303)

DESCRIPTION

General description

Acquired resistance to chemotherapy drugs, multidrug resistance or MDR, is a major contributor to treatment failure for many types of cancers.[1][2] MDR is typically associated with the increased expression of two ATP-dependent drug efflux pumps, P-Glycoprotein (P-gp or MDR1) and the Multidrug Resistance-Associated Protein (MRP1). These pumps actively expel chemotherapeutic agents, typically hydrophobic amphipathic natural products, from the cytoplasm to exterior of the cell.[3]

This kit is utilizes a hydrophobic fluorescent dye molecule to assess MDR activity in cells. This dye rapidly penetrates cell membranes and becomes trapped resulting in an increase in fluorescence intensity (λex = 490/λem = 525 nm). In cells expressing MDR transporters, the dye is rapidly extruded by the transporters, resulting in decreased fluorescence intensity.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

This kit is suitable for the screening of MDR pump inhibitors or for identifying cell lines with high MDR activity.

Principle

This kit is utilizes a hydrophobic fluorescent dye molecule to assess MDR activity in cells. This dye rapidly penetrates cell membranes and becomes trapped resulting in an increase in fluorescence intensity (λEx = 490/λEm = 525 nm). In cells expressing MDR transporters, the dye is rapidly extruded by the transporters, resulting in decreased fluorescence intensity.

SAFETY INFORMATION

Storage Class Code

10 - Combustible liquids