Acid Phosphatase Activity Fluorometric Assay Kit ยี่ห้อ Sigma

PROPERTIES

usage

sufficient for 500 fluorometric tests

detection method

fluorometric

storage temp.

−20°C

Gene Information

human ... ACP1(52) , ACP2(53)

mouse ... ACP1(11431) , ACP2(11432)

rat ... ACP1(24161) , ACP2(24162)

DESCRIPTION

General description

Acid phosphatases (AP) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimal pH of 4-7. Changes in the levels and activities of AP occur in many pathophysiological conditions including prostate cancer and bone disease.[1][2]

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the detection of acid phosphatase in serum and other biological samples.

Principle

The AP Activity Fluorometric Assay Kit provides a simple and direct procedure for measuring AP activity in serum and other samples. AP activity is determined by the conversion of the non-fluorescent 4-methylumbelliferyl phosphate disodium salt (MUP) to the fluorescent product 4-methylumbelliferone (4-MU) (λex = 360/λem = 440 nm), proportional to the AP activity present. One unit of AP is the amount of enzyme that will hydrolyze 1.0 μmole of MUP per minute at 25 °C.