Choline/Acetylcholine Quantification Kit ยี่ห้อ Sigma

PROPERTIES

usage

sufficient for 100 colorimetric or fluorometric tests (for choline assay and 50 tests acetylcholine assay)

detection method

colorimetric

fluorometric

storage temp.

−20°C

DESCRIPTION

General description

Choline is an essential nutrient, commonly grouped with the B complex vitamins, that plays key roles in many biological processes. Choline is a precursor for the synthesis of acetylcholine, a critical neurotransmitter in both the peripheral and central nervous systems.

Application

Choline/Acetylcholine Quantification Kit has been used to quantify the amounts of choline and acetylcholine in samples.[1][2]

Suitability

Suitable for use with cell and tissue culture supernatants as well as serum, plasma, urine, and other biological fluids.

Principle

In this assay, choline concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the choline present. Acetylcholine levels can be determined by adding acetylcholinesterase to the reaction, which hydrolyzes acetylcholine to choline and acetate.