Adp colorimetric/fluorometric assay kit ยี่ห้อ Sigma

PROPERTIES

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric

fluorometric

storage temp.

−20°C

DESCRIPTION

General description

Adenosine diphosphate (ADP) is a nucleoside diphosphate that plays a critical role in energy transfer reactions. ADP is produced from adenosine triphosphate (ATP) via the action of ATPases.[1] ADP also plays a critical role in platelet function. ADP, stored in platelet-dense granules, is released upon platelet activation where it acts on purinergic receptors to mediate intracellular signaling and platelet aggregation.[2] ADP is a key agonist involved in thrombosis and hemostasis.[3]

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the measuerement of ADP in a variety of biological samples including cell and tissue lysates

Principle

In this assay, ADP (adenosine diphosphate) concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the ADP present.