Fructose-6-Phosphate Assay Kit, ยี่ห้อ Sigma

DESCRIPTION

General description

Fructose-6-phosphate (F6P) is a glycolytic pathway intermediate produced by the isomerization of glucose-6-phosphate by phosphoglucose isomerase. F6P is further phosphorylated to fructose 1,6-biphosphate which is subsequently cleaved to glyceraldehyde phosphate and dihydroxyacetone phosphate. F6P can also be shunted from glycolysis to the non-oxidative branch of the pentose phosphate pathway as a means of generative pentose phosphates for nucleotide synthesis. F6P levels are elevated in rapidly proliferating cells such as cancer cells. The Fructose-6-phosphate Assay Kit is a highly sensitive and simple fluorescence-based method of quantifying F6P in a variety of samples.

Application

Fructose-6-Phosphate Assay Kit has been used to measure fructose-6-phosphate (F-6-P) and glucose-6-phosphate (G6P).

Suitability

Suitable for quantifying Fructose-6-Phosphate in a variety of samples

Principle

Fructose-6-phosphate concentration is determined by a coupled enzyme reaction, which results in a fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the fructose-6-phosphate present. Typical sensitivities for this kit are 0.01 to 0.5 nmoles of F6P.