Glucose-6-Phosphate Dehydrogenase Activity Assay Kit, ยี่ห้อ Sigma

DESCRIPTION

General description

Glucose-6-phosphate dehydrogenase (G6PDH) catalyzes the conversion of glucose-6-phosphate to 6-phosphoglucono-δ-lactone, the first and rate-limiting step of the pentose phosphate pathway (PPP). The PPP pathway critical for maintaining the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH) and for the production of pentose sugars. The NADPH produced is critical for redox regulation via the regeneration of GSH and for providing reducing equivalents for fatty acid biosynthesis. Deficiencies in G6PDH predisposes individuals to non-immune hemolytic anemia.

Application

Glucose-6-phosphate dehydrogenase assay kit has been used in the determination of glucose-6-phosphate dehydrogenase (G6PDH) activity and to measure PDH (pyruvate dehydrogenase).

Suitability

Suitable for the measurement of Glucose-6-phosphate dehydrogenase (G6PDH) activity in various tissues and cells.

Principle

In the glucose-6-phosphate dehydrogenase assay kit, glucose-6-phosphate is oxidized to generate a product which is specifically detected by colorimetric (450 nm) assay. The G6PDH Assay Kit can detect G6PDH activity as low as 0.04mU G6PDH per well.