l-Lactic Dehydrogenase from bovine heart Type III ยี่ห้อ Sigma

PROPERTIES

type

Type III

Quality Level

200

form

ammonium sulfate suspension

specific activity

≥500 units/mg protein

UniProt accession no.

P19858

Q5E9B1

foreign activity

pyruvate kinase ≤0.03%

storage temp.

2-8°C

Gene Information

cow ... LDHA(281274) , LDHB(281275)

DESCRIPTION

General description

Lactic dehydrogenase (LDH) catalyzes the conversion of lactate to pyruvate in anaerobic glycolysis. It exists as tetramer and comprises of two subunits (H and M).[1] The LDH of eukaryotes undergo active-site loop gating for their catalytic functionality.[2]

Application

L-Lactic Dehydrogenase from bovine heart has been used as a standard in cytotoxicity assay.[3] It has also been used in glutamic pyruvic transaminase (GPT) assay.[4]

Biochem/physiol Actions

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Unit Definition

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Physical form

Crystalline suspension in 2.1 M (NH4)2SO4 solution, pH 6.0

Analysis Note

Protein determined by biuret

SAFETY INFORMATION

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable