Xylanase Assay Kit (XylX6 Method) ยี่ห้อ Megazyme

Reference code: K-XylX6-2V

SKU: 700004357

Content:

(K-XylX6-1V)

100 assays (manual) / 200 assays (auto-analyser)

(K-XylX6-2V)

200 assays (manual) / 400 assays (auto-analyser)

Shipping Temperature:

Ambient

Storage Temperature:

Short term stability: 2-8^oC,

Long term stability: See individual component labels

Stability:

> 2 years under recommended storage conditions

Analyte:

endo-1,4-β-Xylanase

Assay Format:

Spectrophotometer, Auto-analyser

Detection Method:

Absorbance

Wavelength (nm):

400,

405 

Signal Response:

Increase

Limit of Detection:

5.3 x 10^-4 U/mL

Reproducibility (%):

~ 3%

Total Assay Time:

10 min

Application examples:

Fermentation broths, industrial enzyme preparations, animal feed, biofuels research, barley malt analysis.

Method recognition:

Novel method

The K-XYLX6-1V pack size has been discontinued (read more)

The XylX6 assay reagent for the measurement of endo-xylanase (endo-1,4-β-xylanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-4⁵-glucosyl-xylopentaoside and 2) β-xylosidase. The ketone blocking group prevents any hydrolytic action by the β-xylosidase or other exo-acting glycosidases on the XylX6 substrate. Incubation with an endo-xylanase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-xylosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of XylX6 by the endo-xylanase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).

Need to measure other enzyme activities? View our complete list of assay kits for measurement of enzyme activity.