Pullulanase/Limit-Dextrinase Assay Kit (PullG6 Method) ยี่ห้อ Megazyme

Reference code: K-PullG6

SKU: 700004329

100/200 assays per kit

Content:

100 / 200 assays per kit

Shipping Temperature:

Ambient

Storage Temperature:

Short term stability: 2-8^oC,

Long term stability: See individual component labels

Stability:

> 2 years under recommended storage conditions

Analyte:

Pullulanase/Limit-Dextrinase

Assay Format:

Spectrophotometer

Detection Method:

Absorbance

Wavelength (nm):

400

Signal Response:

Increase

Limit of Detection:

0.18 U/mL for pullulanase preparations (50-fold dilution)

0.01 U/g for limit dextrinase in milled malt

Reproducibility (%):

~ 3%

Total Assay Time:

~ 10 min (Pullanase),

~ 30 min (Limit-Dextrinase)

Application examples:

Assay of microbial pullulanase preparations. Measurement of limit-dextrinase in malt extracts.

Method recognition:

Novel method

PullG6 assay for the measurement of pullulanase employs a water soluble defined substrate, namely 4,6-O-benzylidene-4-nitrophenyl-6³-α-D-maltotriosyl-maltotriose (BPNPG3G3), coupled with the ancillary enzymes α-glucosidase and β-glucosidase. Upon hydrolysis of the substrate at the 1,6-α-linkage by pullulanase or limit-dextrinase, the released 4-nitrophenyl-β-maltotrioside is immediately hydrolysed to glucose and 4-nitrophenol by the concerted action of the α-glucosidase and β-glucosidase enzymes in the reagent mixture. The reaction is terminated and phenolate ions are developed by addition of dilute alkali. The absorbance is read at 400 nm and the value obtained correlates directly with pullulanase activity.