Phosphate Assay Kit ยี่ห้อ Megazyme

Reference code: K-PHOS

SKU: 700004326

100 assays (manual) / 400 assays (auto-analyser)

Content:

100 assays (manual) / 400 assays (auto-analyser)

Shipping Temperature:

Ambient

Storage Temperature:

Short term stability: 2-8^oC,

Long term stability: See individual component labels

Stability:

> 2 years under recommended storage conditions

Analyte:

Phosphate

Assay Format:

Spectrophotometer, Auto-analyser

Detection Method:

Absorbance

Wavelength (nm):

360

Signal Response:

Increase

Linear Range:

0.1 to 10 μg of phosphate per assay

Limit of Detection:

0.16 mg/L

Reaction Time (min):

~ 20 min

Application examples:

Processed foods and drinks, bakery products, dairy products, waste water samples, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, etc.).

The Phosphate Assay Kit is a rapid, simple, reliable and accurate method for the specific measurement and analysis of phosphate in water, foodstuffs, beverages and other materials. The phosphate detection reaction employs the defined chemical substrate 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG). In the presence of phosphate, the substrate is converted enzymatically by purine nucleoside phosphorylase (PNPase) to the products ribose 1-phosphate and 2-amino-6-mercapto-7-methylpurine. An application note describes the dephosphorylation reaction of phytic acid using phytase and alkaline phosphatase (sold separately), and subsequent phosphate detection using the reagents provided in K-PHOS.

This method is suitable for analysis using spectrophotometer and auto-analyser.