Hydrogen Peroxide Assay Kit (Megaplex Red) ยี่ห้อ Megazyme

Reference code: K-MRH2O2

SKU: 700004322

1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser)

Content:

1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser)

Shipping Temperature:

Ambient

Storage Temperature:

Short term stability: 2-8^oC,

Long term stability: See individual component labels

Stability:

> 2 years under recommended storage conditions

Analyte:

Hydrogen Peroxide

Assay Format:

Spectrophotometer, Microplate, Auto-analyser

Detection Method:

Absorbance, Fluorescence

Wavelength (nm):

(Fluorescence) 530-560 (ex), ~ 590 (em)

(Absorbance) 570

Signal Response:

Increase

Linear Range:

(Fluorescence) 0.0034-0.17 µg of H₂O₂ per assay (0.1-5 µM in-assay)

(Absorbance) 0.034-0.68 µg of H₂O₂ per assay (1-20 µM in-assay)

Limit of Detection:

(Fluorescence) 0.0034 ug/mL

(Absorbance) 0.25 ug/mL

Reproducibility (%):

(Fluorescence) ~ 8%

(Absorbance) ~ 8%

Reaction Time (min):

(Fluorescence) 30 min

(Absorbance) 30 min

Application examples:

Food and beverage samples such as milk or milk based products, wine, fruit juices, paper (and cardboard), waste treatment samples and other materials (e.g. biological cultures etc.).

This product has been discontinued.

The hydrogen peroxide Assay Kit provides a simple robust method for the measurement of hydrogen peroxide. This kit utilizes a highly sensitive Megaplex Red probe which in the presence of horseradish peroxidase (HRP), allows for the measurement of H₂O₂ in a sample. Upon oxidation of the probe by HRP, a coloured product (resorufin) is formed that can be measured in fluorescence mode using excitation in the range 530-560 nm and emission at ~ 590 nm or colourimetrically at 570 nm. The excitation and emission maxima of Megaplex Red are 571 nm and 585 nm respectively.