MILLIPLEX® Human Circulating Cancer Biomarker Magnetic Bead Panel - Cancer Multiplex Assay ยี่ห้อ Sigma

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General description

Circulating cancer biomarkers give insight beyond intracellular oncogenesis and into how the body responds to the presence of a tumor, the dysregulation of homeostasis, and the relationship between a tumor and its environment. Multianalyte panels have become essential tools that enable researchers to measure cancer biomarkers simultaneously, teasing out these relationships and applying them to understand tumor biology.

MILLIPLEX^® Circulating Cancer Biomarker Panel 1 is a 25-plex kit that can be used for the simultaneous quantification of any or all of the following analytes in serum, plasma and tissue/cell lysate and culture supernatant samples: AFP, CA125, CA15-3, CA19-9, CEA, CYFRA21-1, sFas, sFasL, FGF2, β-HCG, HE4, HGF, IL-6, IL-8, Leptin, MIF, Osteopontin, Prolactin, PSA (free), PSA (total), SCF, TGFα, TNFα, TRAIL and VEGF. Note that certain biomarkers are tumor specific, such as PSA, while others, such as IL-8, have been detected in many cancers. PSA (free) and PSA (total) cannot be plexed together. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Circulating Cancer

Specificity

Cross Reactivty

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

·      Analytes: α-Fetoprotein (AFP), CA125, CA15-3, CA19-9, CEA, CYFRA21-1, sFas (TNFRSF6), sFasL, FGF-2 (FGF-basic), β-HCG (HCGβ), HE4, HGF, IL-6, IL-8 (CXCL8), Leptin, MIF, Osteopontin (OPN), Prolactin, PSA (free)*, PSA (total)*, SCF, TGFα, TNFα, TRAIL (TNFSF10), VEGF.

·      Note: Total PSA and Free PSA cannot be plexed together

·      Recommended Sample Type: Human serum, plasma or tissue/cell culture supernatants or lysates

·      Recommended Sample Dilution: 25 μL per well of 1:6 diluted plasma or serum, or 25 μL cell culture psaesaa sample undiluted or diluted in appropriate control medium

·      Assay Run Time: Overnight (16-18 hr) at 2-8°C

·      Research Category: Cancer

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

Everything you need in a single kit.

Components

Human Circulating Cancer Biomarker Panel 1 Standard (HCC-8058)

Human Circulating Cancer Biomarker Panel 1 Quality Controls 1 and 2 (HCC-6058)

Serum Matrix (HCP-SM)

Note: Contains 0.08% Sodium Azide

Set of one 96-Well Plate with 2 Sealers (MAG-PLATE)

Assay Buffer (L-AB)

Note: Contains 0.08% Sodium Azide

10X Wash Buffer (L-WB)

Note: Contains 0.05% Proclin

Human Circulating Cancer Biomarker Panel 1 Detection Antibodies (HCC-1058 or HCC-1058-2)

Streptavidin-Phycoerythrin (L-SAPE 4)

Bead Diluent (LBD-4)

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Please note: Total PSA and Free PSA cannot be plexed together.

Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.