HIS-Select® Cobalt Affinity Gel ยี่ห้อ MERCK

PROPERTIES

Quality Level

200

form

(1:1 suspension in a 30% ethanol solution)

shelf life

1 yr (when stored properly.)

matrix

6% Beaded Agarose

capacity

>15 mg/mL, agarose binding capacity (protein)(with an approx. 30 kDa protein)

storage temp.

2-8°C

DESCRIPTION

General description

The HIS-Select Cobalt Affinity Gel is a quadridentate chelate on beaded agarose that is charged with cobalt. The gel is designed to specifically bind histidine-containing proteins / His-tag proteins. It is selective for recombinant proteins with histidine tags and exhibits low non-specific binding of other proteins.

HIS-Select Cobalt Affinity Gel is an immobilized metal-ion affinity chromatography (IMAC) product. The matrix for this affinity gel is 6% beaded agarose. The selectivity can be modulated with the inclusion of imidazole during chromatography. HIS-Select Cobalt Affinity Gel is durable and can capture recombinant proteins with histidine tags at a high flow rate. Recombinant proteins with His tags are bound using native, denaturing, or mild reducing conditions. The capacity of this affinity gel is typically >15 mg/mL of packed gel, determined with a His-tagged protein of molecular mass ~30 kDa.

Application

HIS-Select Cobalt Affinity Gel allows for high purity, low non-specific binding, and high binding capacity of protein. HIS-Select Cobalt Affinity Gel also works well for purification of histidine-tagged proteins / His-tag proteins in native, denaturing, or mild reducing conditions. HIS-Select^™ Cobalt Affinity Gel has been used to purify His-tagged proteins.[1]

Physical form

1:1 suspension in a 30% ethanol solution

Legal Information

HIS-Select is a registered trademark of Sigma-Aldrich Co. LLC

HiScreen is a trademark of Cytiva