Glycine, for electrophoresis, ≥99% ยี่ห้อ Sigma

PROPERTIES

Quality Level

200

Assay

≥99%

form

powder

technique(s)

electrophoresis: suitable

western blot: suitable

impurities

≤0.01% Insoluble matter

color

white to off-white

pKa (25 °C)

(1) 2.35, (2) 9.60

2.35

mp

240 °C (dec.) (lit.)

solubility

H₂O: 200 mg/mL, clear, colorless to faintly yellow

anion traces

chloride (Cl^-): ≤70 ppm

cation traces

heavy metals (as Pb): ≤20 ppm

absorption

≤0.15 at 280 at 1 M

SMILES string

NCC(O)=O

InChI

1S/C2H5NO2/c3-1-2(4)5/h1,3H2,(H,4,5)

InChI key

DHMQDGOQFOQNFH-UHFFFAOYSA-N

Gene Information

rat ... Grin2a(24409)

DESCRIPTION

General description

Glycine is a molecule with pI of 6.7, which is similar to the pH of stacking gel. It has advantage of low mobility, hydrophobicity and it does not associate with proteins.[1] Glycine is a component of Tris-glycine and Tris-glycine-SDS (sodium dodecyl sulfate) running buffers for polyacrylamide gel electrophoresis.[2] Glycine is also a component of Towbin′s transfer buffer for Western blots.[3]

Application

·       Glycine has been used for the preparation of gelatin composites scaffold.[4]

·       It has been used in the elution buffer to extract protein using HiTrap protein G column.[5]

·       It has been used in the SDS-sample, running and transfer buffers prepared for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and western blotting.[6]

Biochem/physiol Actions

Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors.

Analysis Note

Tested for use in electrode buffers for PAGE and in transfer buffers for Western blots.

SAFETY INFORMATION

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable