Human Fc gamma RI / CD64 Protein, His Tag (SPR & BLI & MALS verifie

Synonym

• FCGR1A, FCG1, FCGR1, IGFR1, CD64, CD64A, FCRI

Source

• Human CD64, His Tag(FCA-H52H1) is expressed from human 293 cells (HEK293). It contains AA Gln 16 - Leu 281 (Accession # P12314-1 ).
• Predicted N-terminus: Gln 16

Molecular Characterization

• This protein carries a polyhistidine tag at the C-terminus.
• The protein has a calculated MW of 31.8 kDa. The protein migrates as 45-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

• Less than 1.0 EU per μg by the LAL method.

Purity

• >90% as determined by SDS-PAGE.
• >90% as determined by SEC-MALS.

Formulation

• Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Contact us for customized product form or formulation.

Reconstitution

• Please see Certificate of Analysis for specific instructions.
• For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

• For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Please avoid repeated freeze-thaw cycles.
• This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 12 months under sterile conditions after reconstitution.

SDS-PAGE

Human CD64, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA

Immobilized Ipilimumab at 1 μg/mL (100 μL/well) can bind Human CD64, His Tag (Cat. No. FCA-H52H1) with a linear range of 2-8 ng/mL (QC tested).

Bioactivity-SPR

Human CD64, His Tag (Cat. No. FCA-H52H1) captured on CM5 chip via anti-His antibody can bind Herceptin® with an affinity constant of 4.92 nM as determined in a SPR assay (Biacore 8K) (QC tested).

Bioactivity-BLI

Loaded Human CD64, His Tag (Cat. No. FCA-H52H1) on HIS1K Biosensor, can bind Herceptin with an affinity constant of 6.98 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Loaded Herceptin on FAB2G Biosensor, can bind Human CD64, His Tag (Cat. No. FCA-H52H1) with an affinity constant of 0.244 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

SEC-MALS

The purity of Human CD64, His Tag (Cat. No. FCA-H52H1) is more than 90% and the molecular weight of this protein is around 44-60 kDa verified by SEC-MALS.

Herceptin captured on Protein A Chip can bind Human CD64, His Tag (Cat. No. FCA-H52H1) with an affinity constant of 0.108 nM as determined in SPR assay (Biacore 8K) (Routinely tested).

Loaded Herceptin on Protein A Biosensor, can bind Human CD64, His Tag (Cat. No. FCA-H52H1) with an affinity constant of 0.297 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).