Stress Signaling Pathway Analysis Response Element Firefly Luciferase Vectors

Firefly Luciferase Reporter Vectors with Cellular Stress Response Elements

• Offer a range of response elements that are involved in stress signalling pathways
• Each vector included an optimized firefly luciferase gene that codes a reporter with a one-hour half-life
• Hygromycin resistance selectable marker means choosing transient transfection or generating a stable cell line

Creating a cell line with an indicator of a functional signaling pathway is useful for deciphering the components in a signaling pathway. These tools are made by insertion of multiple repeats of a response element upstream of a minimal promoter (minP). Promega has designed vectors that report the activity of a variety of pathways using the optimized luc2 firefly luciferase gene in the pGL4 backbone. These vectors also have a hygromycin resistance selectable marker, allowing use either in transient transfection experiments or for selection of a stable cell line. In addition, Some of these pre-designed response element vectors also are available stable cell lines (GloResponse™ Cell Lines) to speed your research.

There are more vectors for measuring cytokine signaling pathways and additional signaling pathways.

Applications

• Cell transcription.
• Virus-cell interactions.
• Compound screening.
• Post-translational modifications.
• GPCR signaling.
• Cell signaling.

About the pGL4 Reporter Vectors

The pGL4 Vectors offer increased reporter gene expression with codon optimization of synthetic genes for mammalian expression and reduced background and risk of expression artifacts with removal of cryptic DNA regulatory elements and transcription factor binding sites. Firefly luciferase has options that offer improved temporal response with the destabilized Rapid Response™ luciferase genes.

Sequence Information

pGL4.37[luc2P/ARE/Hygro] Vector GenBank® Accession Number JQ858521 and vector sequence text file.

pGL4.38[luc2P/p53 RE/Hygro] Vector GenBank® Accession Number JQ858522 and vector sequence text file.

pGL4.39[luc2P/ATF6 RE/Hygro] Vector GenBank® Accession Number JQ858519 and vector sequence text file.

pGL4.40[luc2P/MRE/Hygro] Vector GenBank® Accession Number JQ858515 and vector sequence text file.

pGL4.41[luc2P/HSE/Hygro] Vector GenBank® Accession Number JQ858520 and vector sequence text file.

pGL4.42[luc2P/HRE/Hygro] Vector GenBank® Accession Number JQ858518 and vector sequence text file.

pGL4.43[luc2P/XRE/Hygro] Vector GenBank® Accession Number JQ858513 and vector sequence text file.

ItemPart #Size

ConcentrationpGL4.37[luc2P/ARE/Hygro] VectorE364A1 × 20μg1μg/μl

แหล่งที่มา : https://worldwide.promega.com/products/luciferase-assays/genetic-reporter-vectors-and-cell-lines/stress-signaling-pathway-analysis-minimal-promoter-driven-firefly-luciferase-vectors/?catNum=E3641