pmirGLO Dual-Luciferase miRNA Target Expression Vector

Evaluate microRNA Activity by Inserting miRNA Target Sites 3´ of the Firefly Luciferase Gene

• Reporter activity correlates with miRNA activity
• Optimized luc2 reporter gene provides highest expression while the Renilla luciferase gene provides normalization
• The moderate-strength PGK promoter provides more biologically relevant analysis not possible with strong promoters

The pmirGLO Vector is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites downstream or 3´ of the firefly luciferase gene (luc2). Firefly luciferase is the primary reporter gene; reduced firefly luciferase expression indicates the binding of endogenous or introduced miRNAs to the cloned miRNA target sequence. This vector is based on Promega dual-luciferase technology, with luc2 used as the primary reporter to monitor mRNA regulation and Renilla luciferase (hRluc-neo) acting as a control reporter for normalization using the Dual-Luciferase® Assay and selection for stable cell lines.

Applications

• miRNA detection.
• miRNA binding site analysis.
• Transcript stability study.
• 3´-UTR analysis.

Normalized luciferase activity using the pmirGLO Vector with an mi-R21 target sequence.

Normalized luciferase activity using the pmirGLO Vector with an mi-R21 target sequence.

ItemPart #Size

Oligo Annealing Buffer

C838A1 × 1ml

pmirGLO Vector

E133A1 × 20μg

แหล่งที่มา : https://worldwide.promega.com/products/luciferase-assays/genetic-reporter-vectors-and-cell-lines/pmirglo-dual-luciferase-mirna-target-expression-vector/?catNum=E1330