Pectate Lyase (Aspergillus sp.) ยี่ห้อ Megazyme

Reference code: E-PCLYAN2

7,500 Units in 3.2 M ammonium sulphate

Content:

7,500 Units in 3.2 M ammonium sulphate

Shipping Temperature:

Ambient

Storage Temperature:

2-8^oC

Formulation:

In 3.2 M ammonium sulphate

Physical Form:

Suspension

Stability:

> 4 years at 4^oC

Enzyme Activity:

Pectate Lyase

EC Number:

4.2.2.2

CAZy Family:

PL1

CAS Number:

9015-75-2

Synonyms:

pectate lyase; (1,4)-alpha-D-galacturonan lyase

Source:

Aspergillus sp.

Molecular Weight:

45,000

Concentration:

Supplied at ~ 3,000 U/mL

Expression:

From Aspergillus sp.

Specificity:

Eliminative cleavage of (1,4)-α-D-galacturonan to give oligosaccharides with 4-deoxy-α-D-galact-4-enuronosyl groups at their non-reducing ends.

Specific Activity:

~ 180 U/mg (40^oC, pH 8.0 on polygalacturonic acid)

Unit Definition:

One Unit of pectate lyase activity is defined as the amount of enzyme required to release one µmole of galacturonic acid from polygalacturonic acid (2.5 mg/mL) per min in Tris.HCl buffer (50 mM), pH 8.0 at 40^oC.

Temperature Optima:

40^oC

pH Optima:

8

Application examples:

Suitable for pectin identification according to USP method [Pectin 9000-69-5]; Pectin Identification Procedure. Applications in carbohydrate and biofuels research.