Glucoamylase P (H. resinae) ยี่ห้อ Megazyme

Reference code: E-GAMP

340 Units at 40^oC;

~ 800 Units at 60^oC

Content:

340 Units at 40^oC;

~ 800 Units at 60^oC

Shipping Temperature:

Ambient

Storage Temperature:

2-8^oC

Formulation:

In 3.2 M ammonium sulphate

Physical Form:

Suspension

Stability:

> 4 years at 4^oC

Enzyme Activity:

Amyloglucosidase

EC Number:

3.2.1.3

CAZy Family:

GH15

CAS Number:

9032-08-0

Synonyms:

amyloglucosidase; exo-1,4-alpha-glucosidase; glucan 1,4-alpha-glucosidase; glucoamylase

Source:

Hormoconis resinae

Molecular Weight:

65,400

Concentration:

Supplied at ~ 170 U/mL

Expression:

Recombinant from Hormoconis resinae

Specificity:

Hydrolysis of terminal non-reducing α-1,4-D-glycosidic bonds in α-1,4-D-glucans with “debranching activity” (hydrolysis of α-1,6-D-glycosidic bonds) in substrates such as starch and pullulan.

Specific Activity:

~ 60 U/mg (40^oC, pH 4.5 on soluble starch); 

~ 160 U/mg (60^oC, pH 4.5 on soluble starch)

Unit Definition:

One Unit of glucoamylase activity is defined as the amount of enzyme required to release one µmole of β-D-glucose reducing-sugar equivalents per minute from soluble starch (10 mg/mL) in sodium acetate buffer (100 mM), pH 4.5.

Temperature Optima:

60^oC

pH Optima:

4.5

Application examples:

Applications in carbohydrate and biofuels research and in the food and feeds industries.