endo-1,4 β-Mannanase (Cellvibrio japonicus) ยี่ห้อ Megazyme

Reference code: E-BMACJ

10,000 Units

Content:

10,000 Units

Shipping Temperature:

Ambient

Storage Temperature:

2-8^oC

Formulation:

In 3.2 M ammonium sulphate

Physical Form:

Suspension

Stability:

Minimum 1 year at 4^oC. Check vial for details.

Enzyme Activity:

endo-1,4-β-Mannanase

EC Number:

3.2.1.78

CAZy Family:

GH26

CAS Number:

37288-54-3

Synonyms:

mannan endo-1,4-beta-mannosidase; 4-beta-D-mannan mannanohydrolase

Source:

Cellvibrio japonicus

Molecular Weight:

45,200

Concentration:

Supplied at ~ 5,000 U/mL

Expression:

Recombinant from Cellvibrio japonicus

Specificity:

Random hydrolysis of (1,4)-β-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Specific Activity:

~ 250 U/mg (40^oC, pH 7.0 on carob galactomannan);

~ 420 U/mg (50^oC, pH 7.0 on carob galactomannan)

Unit Definition:

One Unit of mannanase activity is defined as the amount of enzyme required to release one µmole of mannose reducing-sugar equivalents per minute from carob galactomannan (5 mg/mL) in sodium phosphate buffer (100 mM), pH 7.0.

Temperature Optima:

50^oC

pH Optima:

7

Application examples:

Applications established in diagnostics and research within the food and feed, carbohydrate, biofuels and paper production industries.