Biotinylated Molecular Weight Marker, mol wt 6,500–180,000 Da ยี่ห้อ Sigma

PROPERTIES

form

lyophilized powder

Quality Level

200

usage

1 vial sufficient for 200 applications (on 10 cm × 10 cm mini gels)

mol wt

6,500-180,000 Da

packaging

vial of 0.1 mg protein (also contains 0.1 mg NaCl and 0.1 mg sucrose)

storage temp.

−20°C

DESCRIPTION

Application

For SDS-PAGE and protein transfer, the Biotinylated Molecular Weight Standard Mixture contains proteins, which have been biotin conjugated for use as SDS-PAGE standards for the estimation of the molecular weights of blotted proteins.[1] Visualization is accomplished using streptavidin-peroxidase (Cat. No. S2438) and a color development reagent such as 4-Chloro-1-naphthol (Cat. No. C8890), eliminating the need to cut out separate lanes for general protein stains and can be performed simultaneously with immunostaining procedures. These standards may be used with either the Laemmli[2] or the Weber and Osborn[3] SDS-PAGE procedures.

Components

This product contains:

·       α₂-macroglobulin (equine serum)

·       β-galactosidase (E. coli)

·       phosphorylase b (rabbit muscle)

·       catalase (bovine liver)

·       alcohol dehydrogenase (yeast)

·       carbonic anhydrase (bovine erythrocyte)

·       trypsin inhibitor (soy bean)

·       lysozyme (chicken egg)

·       aprotinin (bovine lung)

Each vial contains approximately 33% protein, 33% NaCl and 33% sucrose.

Other Notes

Contains nine biotinylated proteins (approx 0.1 mg total).

Reconstitution

Each vial is reconstituted with 1 mL of 1x sample buffer (Cat. No. S3401) and incubated for 2-3 minutes at 100 °C.

SAFETY INFORMATION

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable