Bovine Serum Albumin, dimer mol wt ~132 kDa ยี่ห้อ Sigma

PROPERTIES

biological source

bovine

Quality Level

200

form

lyophilized powder

mol wt

dimer ~132 kDa

monomer ~66 kDa

~66 kDa

purified by

cold ethanol fractionation

origin

USA origin

pH

5.2

storage temp.

−20°C

DESCRIPTION

Application

Marker for non-denaturing PAGE

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Reconstitution

0.9-1.4 mg/mL after reconstitution with 1 mL of water

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SAFETY INFORMATION

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable