Bovine Serum Albumin, heat shock fraction, pH 7, ≥98% ยี่ห้อ Sigma

PROPERTIES

biological source

bovine

Quality Level

300

Assay

≥98%

form

lyophilized powder

mol wt

~66 kDa

purified by

heat shock fractionation

origin

USA origin

technique(s)

blood typing: suitable

microbiological culture: suitable

loss

≤5%

pH

7

solubility

water: soluble (40 mg/ml)

UniProt accession no.

P02769

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

DESCRIPTION

Application

Bovine serum albumin has been used-

·       as a component of PCR (polymerase chain reaction) mix[1]

·       in the preparation of dissociated cortical neurons[2]

·       during immunofluorescent labeling[2]

·       as a component of Ca²⁺-free saline (containing collagenase, hyaluronidase and DNAse) used for bovine tissue digestion[3]

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Prepared using heat shock fractionation.

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

SAFETY INFORMATION

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable