Acrylamide, for electrophoresis, ≥99% (HPLC), powder ยี่ห้อ MERCK

PROPERTIES

vapor density

2.45 (vs air)

Quality Level

200

vapor pressure

0.03 mmHg ( 40 °C)

Assay

≥99% (HPLC)

form

powder

technique(s)

electrophoresis: suitable

impurities

≤0.005% Acrylic acid

≤0.005% Insoluble in water

conductivity

≤10 μmho (35% solution)

bp

125 °C/25 mmHg (lit.)

mp

82-86 °C (lit.)

solubility

water: soluble 2.5 g/10 mL, clear, colorless

cation traces

Fe: ≤1 ppm

Mg: ≤3 ppm

heavy metals (as Pb): ≤5 ppm

SMILES string

NC(=O)C=C

InChI

1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)

InChI key

HRPVXLWXLXDGHG-UHFFFAOYSA-N

DESCRIPTION

General description

Acrylamide functions as a quencher of tryptophan fluorescence.[1] Acrylamide along with bis-acrylamide crosslinks to form polyacrylamide gels,[2] which is used to separate protein and other biomolecules. Bis-acrylamide is essential for crosslinking and acrylamide is required for the polymerization of monomers.[2] Acrylamide alkylates SH groups of cysteine and ε-NH₂ lysine residues of proteins during electrophoresis.[1]

Application

Acrylamide has been used in the preparation of polyacrylamide gels.[3][4]

Compatibility

This substance has been tested against several types of hand protection for CE compliance. See Related Products to find the recommended gloves for handling this product.