Wizard® HMW DNA Extraction Kit

Purify High-Molecular-Weight DNA for Long-Read Sequencing

• Optimized protocol for extracting high-purity HMW DNA
• Reliable performance on long-read systems such as the Oxford Nanopore Technologies® instruments
• Rapid protocol with no hazardous chemicals

High-Quality High-Molecular-Weight DNA from a Variety of Sample Types

De novo genome sequencing, genome finishing and structural variant detection have become significantly more commonplace due to advances in sequencing technology. Long-read sequencing in particular provides researchers with data to address challenges in fields such as oncology, inherited disease research, microbiology and agriculture. However, long-read technologies require high-quality, high-molecular-weight DNA. Obtaining DNA of sufficient, size, quantity and quality is critical to the process that has historically required costly or laborious methods.

The Wizard® HMW DNA Extraction Kit is specifically designed to isolate high-molecular-weight (HMW) DNA that will provide strong performance in long-read sequencing. This kit helps researchers obtain DNA up to 500kb with high purity to get the most out of precious samples and can be used with a wide range of sample types, including whole blood, plant leaf, tissue culture cells and Gram-positive and Gram-negative bacteria. With a workflow that can be completed in approximately 1.5 hours without specialized equipment, the Wizard® HMW DNA Extraction Kit relieves many of the challenges regularly faced by researchers.

ItemPart #SizeAvailable Separately

HMW Blood Lysis BufferA293A1 × 50ml

HMW Lysis Buffer AA294A1 × 25ml

Protein Precipitation SolutionA795A1 × 25mlView Product

DNA Rehydration SolutionA796A1 × 50mlView Product

RNase A SolutionA797A1 × 250μlView Product

Proteinase K (PK) SolutionMC500C1 × 1mlView Product

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