ReliaPrep™ Large Volume HT gDNA Isolation System

Automated DNA Isolation from 1–10ml Blood

• Effective recovery from pristine, hemolysed or frozen samples
• Purified DNA ready for use in downstream assays or archiving
• Chemistry scaled for each sample, reducing waste

Scalable, Walkaway DNA Purification from Large-Volume Blood Samples

The ReliaPrep™ Large Volume HT gDNA Isolation System is used with the HSM 2.0 Instrument (Cat.# A2715) to provide a scalable, efficient method for isolating genomic DNA (gDNA) from 1–10ml blood samples. The precipitation-free ReliaPrep™ DNA purification chemistry eliminates the tedious centrifugation steps and hazardous chemicals associated with precipitation-based methods, reducing purification failures. Each kit provides enough reagents to process up to 96 × 10ml whole blood samples. 

The system has been automated on robotic liquid-handling workstations, allowing walkaway purification of 1–10ml whole blood, regardless of sample storage or shipping conditions.

Genomic DNA yields from 10ml normal whole blood samples are typically 200–400µg (depending on white blood cell count) in a final eluted volume of 1ml. Recovered DNA exhibits good purity with A₂₆₀/A₂₈₀ ratios greater than 1.7 and A₂₆₀/A₂₃₀ ratios between 1.8 and 2.2.

Products may not be available in all countries.

Download our Green Sheet to learn more about the features that make this an Environmentally Preferable Product:

ItemPart #SizeAvailable Separately

Alkaline Protease (APA)A17211 × 130mlView Product

Cell Lysis Buffer (CLD)A17311 × 1,400mlView Product

Binding Buffer (BBA)A17411 × 1,600mlView Product

ReliaPrep™ ResinA17521 × 115mlView Product

Prepared Wash Buffer (WBC)A26811 × 3,500mlView Product

Proteinase K (PK) SolutionA50511 × 23mlView Product

Nuclease-Free WaterP11954 × 150mlView Product

แหล่งที่มา : https://worldwide.promega.com/products/nucleic-acid-extraction/genomic-dna/reliaprep-large-volume-ht-gdna-isolation-system/?catNum=A2751