PureYield(TM) Plasmid Midiprep System, 25 preps ยี่ห้อ Promega (Stroge. Temp +15C TO +30C)
รหัสสินค้า : A2492
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PureYield™ Plasmid Midiprep System
Up to 200µg of Transfection-Ready Plasmid DNA from 50ml Cultures
- 30-minute, vacuum-based protocol
- Highly pure, concentrated plasmid DNA
- No tedious, gravity-drip columns or post-elution alcohol precipitation
The PureYield™ Plasmid Midiprep System provides a rapid method for purification of 100–200μg of transfection-quality plasmid DNA from 50ml bacteria cultures. Using the vacuum protocol, plasmid isolation is complete in as little as 30 minutes, significantly reducing time spent on purification compared to silica resin or other membrane-column methods. An alternative protocol allows purification of over 400μg of high-copy-number plasmid from 250ml of E. coli culture.
The system incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves performance in sensitive applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation. Purification is achieved without isopropanol precipitation of purified plasmid DNA or extensive centrifugation.
PureYield™ Plasmid Midipreps can be used with centrifugation or vacuum-based protocols.
ItemPart #SizeAvailable Separately
Neutralization Solution (NSB)A148H1 × 130mlView Product
PureYield™ Binding ColumnsA245A5 × 5 each
PureYield™ Clearing ColumnsA246A5 × 5 each
Endotoxin Removal WashA251C1 × 85.3ml
Column WashA252C1 × 210ml
Cell Resuspension Solution (CRA)A711F1 × 75mlView Product
Cell Lysis Solution (CLA)A712F1 × 75mlView Product
Nuclease-Free WaterP119C1 × 25mlView Product
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