PureYield™ Plasmid Maxiprep System

Up to 1mg of Transfection-Ready Plasmid from 250ml Cultures

• One-hour, vacuum-based protocol
• Highly pure, concentrated plasmid DNA
• No tedious, gravity-drip columns or post-elution alcohol precipitation

The PureYield™ Plasmid Maxiprep System purifies up to 1mg of transfection-quality plasmid DNA from 250ml bacterial cultures in under an hour. The system is designed for use with a vacuum source and vacuum manifold, greatly reducing the time spent on purification compared to silica resin or other membrane-based methods.

PureYield™ Plasmid Maxipreps include a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants generates highly pure DNA with improved performance in sensitive applications such as eukaryotic transfection, in vitro transcription and transcription/translation applications. Purification is achieved without isopropanol precipitation of purified plasmid DNA.

Advantages of the PureYield™ Plasmid Maxipreps System:

• Simplifies purification of multiple samples at one time.
• Eliminates tedious, gravity-drip columns and post-elution alcohol precipitation.
• Purifies large amounts of plasmid DNA, exceeding the capabilities of other maxiprep systems.

This product requires the use of a vacuum pump and vacuum manifold.

ItemPart #SizeAvailable Separately

Neutralization Solution (NSB)A148J1 × 315mlView Product

PureYield™ Clearing ColumnsA246A5 × 5 each

PureYield™ Maxi Binding ColumnsA248A5 × 5 each

Endotoxin Removal WashA251C1 × 85.3ml

Column WashA252C1 × 210ml

Cell Resuspension Solution (CRA)A71151 × 315ml

Cell Lysis Solution (CLA)A71251 × 315ml

Nuclease-Free WaterP119C2 × 25mlView Product

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