Wizard® SV 96 and SV 9600 Plasmid DNA Purification Systems

Silica Membrane-Based, 96-Well, High-Throughput Plasmid Purification

• 60-minute protocol
• Manual and automated formats
• Vacuum-based method

Simple, Reliable Plasmid Purification

The Wizard® SV 96 and SV 9600 Plasmid DNA Purification Systems provide a simple and reliable method for the rapid isolation of plasmid DNA using a silica-membrane, 96-well, high-throughput format. A single plate can be processed in 60 minutes or less. The Vac-Man® 96 Vacuum Manifold eliminates waste handling and allows simultaneous lysate clearing and DNA binding, and the plate design prevents cross-contamination during sample processing.

Purified plasmid can be used directly for automated fluorescent DNA sequencing as well as for other standard molecular biology techniques. Purified DNA meets a target of >98% accuracy over 600 bases using pGEM®-3Zf(+) Vector DNA in BigDye® terminator sequencing.

The Wizard® SV 96 and SV 9600 Systems are designed for use either in a manual format or with Beckman Coulter or PerkinElmer automated instruments.

The Wizard® SV 96 and Wizard® SV 9600 Systems require a vacuum pump capable of generating 15–20 inches of Hg or equivalent and the Vac-Man® 96 Vacuum Manifold (Cat.# A2291) or similar manifold. The Vac-Man® 96 Vacuum Manifold requires the use of an external vacuum trap (not provided).

ItemPart #SizeAvailable Separately

Alkaline Protease SolutionA144B1 × 3mlView Product

Wizard® SV 96 Neutralization SolutionA148D1 × 84mlView Product

Wizard® SV 96 Lysate Clearing PlateA224A1 × 1 plateView Product

96-Well Deep Well PlateA226A1 × 1 plate

Binding PlateA227A1 × 1 plateView Product

Elution PlateA228A1 × 1 plate

Plate SealersA230A1 × 3 each

Wizard® SV 96 Cell Resuspension SolutionA711U1 × 40mlView Product

Wizard® SV 96 Cell Lysis SolutionA712U1 × 40mlView Product

Nuclease-Free WaterP119E1 × 13mlView Product

Column Wash Solution (CWA)A131C1 × 100mlView Product

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