PureYield(TM) Plasmid Miniprep System, 250 preps ยี่ห้อ Promega (Stroge. Temp +15C TO +30C)
รหัสสินค้า : A1222
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Up to 15μg of Transfection-Ready Plasmid from 3ml Cultures
- Rapid, 10-minute miniprep protocol
- Highly pure, concentrated plasmid DNA
- No tedious, gravity-drip columns or post-elution alcohol precipitation
Size 250 preps
Plasmid Minipreps in Minutes
The PureYield™ Plasmid Miniprep System isolates up to 15μg of transfection-quality plasmid DNA from 600μl to 3ml of bacterial culture in 10 minutes. The system incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves the performance in applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation. Purification is achieved without isopropanol precipitation of purified plasmid DNA or extensive centrifugation, providing rapid purification from a single method.
The PureYield™ Plasmid Miniprep System can be used with centrifugation or vacuum-based protocols, and includes a convenient lysis/neutralization indicator dye.
This miniprep kit can be used to isolate any plasmid from E. coli hosts but works most efficiently when the plasmid is up to 20,000bp in size. For best results, use plasmids that are 10,000bp or less. Purified plasmid can be used without further manipulation for automated fluorescent DNA sequencing as well as for other standard molecular biology techniques. When used for in vitro transcription reactions, the isolated plasmid DNA should be supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511).
ItemPart #SizeCell Lysis Buffer (CLC)
A145C1 × 27mlNeutralization Solution (NSC)
A157C1 × 90mlEndotoxin Removal Wash (ERB)
A158C2 × 27mlColumn Wash Solution (CWC)
A159C1 × 25mlElution Buffer (EBB)
A160C1 × 10mlPureYield™ Minicolumns
A166B5 × 50 columnsPureYield™ Collection Tubes
A167B5 × 50 tubes
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