NucBuster™ Protein Extraction Reagent ยี่ห้อ Sigma

PROPERTIES

grade

for luminescence

for molecular biology

Quality Level

200

product line

BioUltra

Assay

≥99.0% (NT)

form

solid

autoignition temp.

1112 °F

impurities

DNases, none detected

RNases, none detected

insoluble matter, passes filter test

phosphatases, none detected

proteases, none detected

loss

≤0.5% loss on drying, 150 °C

pH

8.5-10.0 (25 °C, 3 M in H₂O)

pKa 

4.76 (acetic acid)

mp

>300 °C (dec.) (lit.)

solubility

H₂O: 3 M at 20 °C, clear, colorless

anion traces

chloride (Cl^-): ≤20 mg/kg

phosphate (PO₄^3-): ≤5 mg/kg

sulfate (SO₄^2-): ≤20 mg/kg

cation traces

Al: ≤5 mg/kg

As: ≤0.1 mg/kg

Ba: ≤10 mg/kg

Bi: ≤5 mg/kg

Ca: ≤10 mg/kg

Cd: ≤5 mg/kg

Co: ≤5 mg/kg

Cr: ≤5 mg/kg

Cu: ≤5 mg/kg

Fe: ≤5 mg/kg

K: ≤200 mg/kg

Li: ≤5 mg/kg

Mg: ≤5 mg/kg

Mn: ≤5 mg/kg

Mo: ≤5 mg/kg

Ni: ≤5 mg/kg

Pb: ≤5 mg/kg

Sr: ≤10 mg/kg

Zn: ≤5 mg/kg

SMILES string

[Na+].CC([O-])=O

λ

3 M in H₂O

UV absorption

λ: 260 nm A_max: 0.03

λ: 280 nm A_max: 0.02

InChI

1S/C2H4O2.Na/c1-2(3)4;/h1H3,(H,3,4);/q;+1/p-1

InChI key

VMHLLURERBWHNL-UHFFFAOYSA-M

DESCRIPTION

Other Notes

Extraction buffer component in the large-scale phenol extraction of RNA (for mRNA isolation)[1].

SAFETY INFORMATION

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable