Glucose-6-Phosphate Dehydrogenase Assay Kit ยี่ห้อ Sigma

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General description

Glucose-6-Phosphate Dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia.

Application

The Glucose-6-Phosphate Dehydrogenase Assay Kit may be used for:

·      Metabolism Research

·      Drug Discovery

Features and Benefits

·      Efficient and Precise: Requires only 20 μL of sample and a short 15-minute reaction time while detecting glucose-6-phosphate dehydrogenase levels across a wide range from 0.2 to 100 U/L, offering both convenience and sensitivity.

·      Compatibility with High-Throughput Systems: Effortlessly incorporate our kit into high-throughput handling systems, guaranteeing seamless integration and maintaining efficiency and accuracy in your laboratory operations.

Suitability

The kit is suitable for the quantitative determination of Glucose-6-Phosphate concentration and evaluation of drug effects on its metabolism in plasma, serum, tissue and culture media, etc.

Principle

The non-radioactive, colorimetric Glucose-6-Phosphate Dehydrogenase Assay Kit is based on the reduction of tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at A₅₆₅ nm. The increase in absorbance at A565 nm is proportional to the enzyme activity.

Other Notes

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