HisPrep™ Fast Flow 16/10 Column ยี่ห้อ MERCK

PROPERTIES

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 28-9365-51

parameter

<10 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)

1.5 bar (22 psi) (Over the Packed Bed During Operation)

22 psi

bed size

16 mm × 100 mm

bed volume

20 mL

column I.D.

16 mm

matrix

6% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning

2-14(Ni²⁺-stripped medium.)

working range

3-12(Ni²⁺-stripped medium.)

capacity

~40 mg binding capacity(histidine-tagged protein)

suitability

suitable for bioprocess medium

DESCRIPTION

General description

HisPrep^™ FF 16/10 is a ready-to-use column, prepacked with precharged Ni Sepharose^® 6 Fast Flow. The column is an excellent choice for preparative, scale-up purification of histidine-tagged recombinant proteins.

HisPrep^™ FF 16/10 columns are prepacked with Ni Sepharose^® 6 Fast Flow for scale-up purification of histidine-tagged proteins. Ni Sepharose^® 6 Fast Flow consists of 90 ?m beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni²⁺ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni²⁺ ions ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.

Features and Benefits

·       Optimized for convenient scale-up purification of histidine-tagged proteins.

·       Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.

·       Negligible leakage of Ni²⁺.

·       High binding capacity, approx. 40 mg/mL medium.

·       Convenient and time-saving 20 mL prepacked HiPrep^™ format.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiPrep is a trademark of Cytiva

HisPrep is a trademark of Cytiva

Sepharose is a registered trademark of Cytiva